False Positives

[ Quality Control/Quality Assurance Main | False Negatives ]

Problem	Control Measures
Artifact PCR products from templates other than the target sequence in question (worst scenario: artifacts of the same size as the expected PCR product)	During validation: Data base research for potential unwanted (homologous) annealing sites Test new primer system on a wide range of types of DNA that could appear in food samples Verify the identity of the PCR product by Southern blot or restriction enzyme digestion
Corruption of sample, extracted sample DNA or PCR setup with positive DNA	During routine analysis: Follow strict basic rules how to work cleanly in a molecular biology lab One way sample flow lab design to prevent contamination with positive DNA from down-stream steps (especially PCR products), strict separation of certain lab areas from each other Duplicate analysis of each sample Negative controls on homogenization, DNA extraction and PCR setup

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